中南大学湘雅二医院耳鼻咽喉头颈外科, 长沙 410011
adgrf3a基因编码的ADGRF3A蛋白是黏附类G蛋白偶联受体(aGPCRs)家族的一员, 主要在受精后5 d的胚胎以及成年斑马鱼的头部和性腺中表达。它含有一个GPS蛋白水解位点和7个跨膜结构域, 与G蛋白相互作用行使其功能。斑马鱼是研究早期发育和成体内生理和病理的重要模式生物。为了研究adgrf3a在斑马鱼早期发育中的作用, 我们利用CRISPR-Cas9技术构建了adgrf3a基因敲除斑马鱼品系。首先, 通过分析软件筛选出该基因的敲除位点, 利用聚合酶链式反应扩增该基因的向导DNA(sgDNA), 再以sgDNA为模板进行体外转录得到向导RNA(sgRNA)并纯化回收, 将纯化后的sgRNA和Cas9蛋白共同注射到斑马鱼1细胞期胚胎中, 得到F0代嵌合体。随后, 对斑马鱼胚胎进行基因编辑的有效性检测, 结果表明, 注射的胚胎出现了碱基缺失的现象, 即sgRNA有效, 将剩余胚胎培养至成鱼。将嵌合体与野生型斑马鱼杂交所得的F1代斑马鱼进行基因型鉴定, 筛选adgrf3a突变杂合子, 并对其adgrf3a突变位点进行Sanger测序, 建立能够稳定遗传的adgrf3a基因杂合突变品系。之后, adgrf3a突变杂合子斑马鱼自交, 获得adgrf3a纯合子突变斑马鱼。体视显微镜观察其成像发现, adgrf3a突变纯合子斑马鱼与野生型整体上未出现明显差异, 然而, 体内组织与器官的发育是否发生变化需要进一步验证。该基因敲除品系的建立为研究adgrf3a在早期发育及病理过程中的作用奠定了基础。
斑马鱼 基因敲除 基因杂合突变品系 zebrafish adgrf3a adgrf3a CRISPR-Cas9 CRISPR-Cas9 gene knockout gene heterozygous mutant strain
1 State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, China
2 Guangzhou New Vision Opto-Electronic Technology Co. Ltd., Guangzhou 510530, China
This paper presents a new compensation pixel circuit suitable for active-matrix organic light-emitting diode (AMOLED) stereoscopic three dimensional (3D) displays with shutter glasses. The simultaneous emission method was used to solve the crosstalk problem, in which the periods of initialization and threshold voltage detection occur for each pixel of whole panel simultaneously. Furthermore, there was no need of the periods of initialization and threshold voltage detection from the second frame beginning by employing threshold voltage one-time detection method. The non-uniformity of the proposed pixel circuit was considerably low with an average value of 8.6% measured from 20 discrete proposed pixel circuits integrated by In-Zn-O thin film transistors (IZO TFTs). It was shown that the OLED current almost remains constant for the number of frames up to 70 even the threshold voltage detection period only exists in the first frame.
active-matrix organic light-emitting diode (AMOLED active-matrix organic light-emitting diode (AMOLED compensation pixel circuit compensation pixel circuit three dimensional (3D) display three dimensional (3D) display simultaneous emission simultaneous emission Frontiers of Optoelectronics
2017, 10(1): 45
1 华南理工大学 电子与信息学院,广东 广州 510640
2 华南理工大学 发光材料与器件国家重点实验室,广东 广州 510641
为方便对AM OLED显示屏的各项性能进行测试,本文设计了基于labVIEW(Laboratory Virtual Instrument Engineering Workbench实验室虚拟仪器工程平台)的AMOLED显示屏VIL曲线自动化测试系统,实现了在同一个软件下对显示屏的电压、电流、亮度、色坐标等特性的测试。首先,构建硬件电路即基于FPGA的AMOLED显示屏驱动电路。接着,在此基础上利用labVIEW平台设计了AMOLED显示屏VIL曲线测试系统的软件系统。最后,利用该系统对自主研发的基于MOTFT技术的122 cm(48 in)AMOLED显示屏进行了测试,验证了系统的可靠性。通过对测试结果进行分析,AMOLED显示屏达到设计亮度时各像素所需的数据电压约为92 V。总之,本系统实现了对显示屏性能参数的自动化测试,为显示屏的白平衡调节和AMOLED专用驱动芯片设计提供了数据支持,为显示屏的优化设计指明方向。
测试 AM OLED AM OLED test labVIEW labVIEW